Anti-LIPG antibody
| Name | Anti-LIPG antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab24447 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Sheep, Human, Pig |
| Antigen | Synthetic peptide surrounding amino acid 22 of human Endothelial Lipase |
| Blocking Peptide | LIPG peptide |
| Description | Rabbit Polyclonal |
| Gene | LIPG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-LIPG antibody (ab24447) + HepG2 lysateObserved band size : 57 kDa (why is the actual band size different from the predicted?)
ICC/IF image of ab24447 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24447, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.