Immunoprecipitation of 293T whole cell lysate using ab191180 (lane 1) at 6µg per reaction (0.5 or 1 mg/IP; 20% of IP loaded/lane). Immunoblot analysis of Lipolysis Stimulated Lipoprotein Receptor utilised ab191180 at 0.4 µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection utilised chemiluminescence with an exposure time of 30seconds.
All lanes : Anti-Lipolysis Stimulated Lipoprotein Receptor antibody (ab191180) at 0.1 µg/mlLane 1 : HeLa whole cell lysate prepared using RIPA lysis buffer.Lane 2 : 293T whole cell lysate prepared using RIPA lysis buffer.Lane 3 : Jurkat whole cell lysate prepared using RIPA lysis buffer.Lysates/proteins at 50 µg per lane.developed using the ECL technique