![Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution + HepG2 cell lysate at 30 µgSecondarygoat anti-mouse HRPdeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/16616_liver-FABP-Primary-antibodies-ab82157-2.jpg)
Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution + HepG2 cell lysate at 30 µgSecondarygoat anti-mouse HRPdeveloped using the ECL technique
ab82157 at 1/100 dilution staining liver FABP in human colon cancer by Immunohistochemistry, Paraffin-embedded tissue detected using Diaminobenzidine (DAB).
![Overlay histogram showing HepG2 cells stained with ab82157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82157, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/16618_liver-FABP-Primary-antibodies-ab82157-5.jpg)
Overlay histogram showing HepG2 cells stained with ab82157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82157, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.