ab135734 staining LMX1b in mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM citrate, pH 6.0. Samples were incubated with primary antibody (1/200 in 1X PBS + 2% BSA + 0.2% Trition X-100) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody. Costaining with 10µg/ml Hoechst 33342 for 1 minute at 25°C.See Abreview
Anti-LMX1b antibody (ab135734) at 1/100 dilution + mouse heart tissue lysates at 35 µg
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labelling LMX1b with ab135734 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining
All lanes : Anti-LMX1b antibody (ab135734) at 1/100 dilutionLane 1 : 293 cell lysates, non-transfected Lane 2 : 293 cell lysates, transiently transfected with the LMX1B geneLysates/proteins at 2 µg per lane.