Anti-LOXL1 antibody
| Name | Anti-LOXL1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab81488 |
| Prices | $378.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Horse, Guinea Pig, Cat |
| Antigen | Synthetic peptide: RWRQLIQWEN NGQVYSLLNS GSEYVPAGPQ RSESSSRVLL AGAPQAQQRR , corresponding to a region within N terminal amino acids 38 - 87 of Human LOXL1 (NP_005567) Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | LOXL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-LOXL1 antibody (ab81488) at 1 µg/ml + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
![LOXL1 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to LOXL1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab81488.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 63kDa; LOXL1](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/17057_LOXL1-Primary-antibodies-ab81488-2.jpg)
LOXL1 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to LOXL1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab81488.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 63kDa; LOXL1