All lanes : Anti-LRG1 antibody (ab128880) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : Human Plasma Total Protein LysateLane 3 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 4 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)Lane 5 : Lung (Human) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.