Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122013 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-LRP5L antibody (ab122013) at 1/250 dilutionLane 1 : Vector only transfected HEK293T lysateLane 2 : LRP5L (co-expressed with a C-terminal myc-DDK tag ~3.1 kDa) transfected HEK293T lysate developed using the ECL technique
ab122013, at a 1/10 dilution, staining Human LRP5L in skeletal muscle, using Immunohistochemistry, Formalin/PFA-fixed parraffin-embedded tissue. Moderate cytoplasmic positivity in myocytes shown.