Anti-LRPAP1 antibody [EPR3327]

• Supplier: Abcam
• Catalog: ab76500
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3327
• Applications: IP IHC-P WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human LRPAP1 aa 1-100
• Description: Rabbit Monoclonal
• Gene: LRPAP1
• Conjugate: Unconjugated

Product images

All lanes : Anti-LRPAP1 antibody [EPR3327] (ab76500) at 1/6000 dilution (unpurified)Lane 1 : Human fetal kidney tissue lysateLane 2 : Mouse kidney tissue lysateLane 3 : Rat kidney tissue lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

All lanes : Anti-LRPAP1 antibody [EPR3327] (ab76500) at 1/3000 dilution (purified)Lane 1 : Human fetal kidney tissue lysateLane 2 : Mouse kidney tissue lysateLane 3 : Rat kidney tissue lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling LRPAP1 with unpurified ab76500 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling LRPAP1 with purified ab76500 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling LRPAP1 with unpurified ab76500 at a 1/100 dilution.

ab76500 (unpurified) at 1/300 immunoprecipitating LRPAP1 in human fetal kidney tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

ab76500 (purified) at 1/150 immunoprecipitating LRPAP1 in human fetal kidney tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.