All lanes : Anti-LSM12 antibody [EPR12282] (ab173291) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HepG2 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.
Immunofluorescent analysis of HepG2 cells labeling LSM12 with ab173291 at 1/100 dilution.
Flow cytometry analysis of permeabilized 293T cells labeling LSM12 with ab173291 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
Western blot analysis on immunoprecipitation pellet from 1) HepG2 cell lysate or 2) 1X PBS (negative control) using ab173291 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.