Flow cytometry analysis of T47-D cells using ab184568 at a 1/110 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
Immunofluorescence analysis of 4% paraformaldehyde fixed T47-D cells labeling LSM5 with ab184568 at a 1/500 dilution. Goat anti rabbit IgG (Alexa Fluor®555) secondary used at a 1/200 diution. Conterstained with Dapi (right image).
Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling LSM5 with ab184568 a 1/100 dilution. Counterstained with Hematoxylin.
Immunohistochemical analysis of paraffin embedded Human astrocytoma tissue labeling LSM5 with ab184568 at a 1/100 dilution. Counterstained with Hematoxylin.
Anti-LSM5 antibody [EPR10690] (ab184568) at 1/10000 dilution + T47-D lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution