Anti-LTBP1 antibody
| Name | Anti-LTBP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab78294 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1350 - 1450 of Human LTBP1 |
| Description | Rabbit Polyclonal |
| Gene | LTBP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/mlLane 1 : Liver (Rat) Tissue Lysate Lane 2 : Pancreas (Mouse) Tissue LysateLane 3 : Spleen (Mouse) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of LTBP1 staining in Mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78294, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/mlLane 1 : Liver (Rat) Tissue LysateLane 2 : Spleen (Mouse) Tissue LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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Maurya VK, Jha RK, Kumar V, Joshi A, Chadchan S, Mohan JJ, Laloraya M. Biol Reprod. 2013 Oct 10;89(4):84.
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Squadrito ML, Pucci F, Magri L, Moi D, Gilfillan GD, Ranghetti A, Casazza A, Mazzone M, Lyle R, Naldini L, De Palma M. Cell Rep. 2012 Feb 23;1(2):141-54.