ab16094 at 1ug/ml detecting LTBR from HEK 293T cells by FACS analysis Thin line: mock transfected HEK 293T cells (5x105)Thick line: HEK 293T cells (5x105) transfected with an expression plasmid enabling surface expression of the extracellular domain of human LTβR Cells were incubated on ice for 30 minutes in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of ab16094. After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 minutes, washed and then analyzed by flow cytometry.