Anti-LXR alpha antibody [PPZ0412] - ChIP Grade
| Name | Anti-LXR alpha antibody [PPZ0412] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab41902 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | PPZ0412 |
| Applications | ChIP ICC/IF ICC/IF WB ELISA IP EMSA IHC-P FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | E |
| Description | Mouse Monoclonal |
| Gene | NR1H3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab41902 staining LXR alpha in Rat Hepatocyte and Kupffer cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
ab41902 staining LXR alpha in rat spleen macrophage cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
![All lanes : Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) at 1/1000 dilution (in 2.5% BSA + TBS for 16 hours at 4°C)Lane 1 : RAW 264.7 murine macrophage cell line lysate without endogenous LXRalphaLane 2 : RAW 264.7 murine macrophage cell line lysate overexpressing human FLAG-LXRalphaLysates/proteins at 35 µg per lane.SecondaryAn HRP-conjugated Goat anti-mouse IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/17908_LXR-alpha-Primary-antibodies-ab41902-4.jpg)
All lanes : Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) at 1/1000 dilution (in 2.5% BSA + TBS for 16 hours at 4°C)Lane 1 : RAW 264.7 murine macrophage cell line lysate without endogenous LXRalphaLane 2 : RAW 264.7 murine macrophage cell line lysate overexpressing human FLAG-LXRalphaLysates/proteins at 35 µg per lane.SecondaryAn HRP-conjugated Goat anti-mouse IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing HepG2 cells stained with ab41902 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41902, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/17909_LXR-alpha-Primary-antibodies-ab41902-8.jpg)
Overlay histogram showing HepG2 cells stained with ab41902 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41902, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Retinoic acid isomers facilitate apolipoprotein E production and lipidation in - Retinoic acid isomers facilitate apolipoprotein E production and lipidation in
Zhao J, Fu Y, Liu CC, Shinohara M, Nielsen HM, Dong Q, Kanekiyo T, Bu G. J Biol Chem. 2014 Apr 18;289(16):11282-92.
miR-206 controls LXRalpha expression and promotes LXR-mediated cholesterol efflux - miR-206 controls LXRalpha expression and promotes LXR-mediated cholesterol efflux
Vinod M, Chennamsetty I, Colin S, Belloy L, De Paoli F, Schaider H, Graier WF, Frank S, Kratky D, Staels B, Chinetti-Gbaguidi G, Kostner GM. Biochim Biophys Acta. 2014 Jun;1841(6):827-35.
Angiotensin-(1-7) upregulates expression of adenosine triphosphate-binding - Angiotensin-(1-7) upregulates expression of adenosine triphosphate-binding
Liang B, Wang X, Bian Y, Yang H, Liu M, Bai R, Yang Z, Xiao C. Clin Exp Pharmacol Physiol. 2014 Dec;41(12):1023-30. doi:
Foam cell specific LXRalpha ligand. - Foam cell specific LXRalpha ligand.
Feldmann R, Geikowski A, Weidner C, Witzke A, Kodelja V, Schwarz T, Gabriel M, Erker T, Sauer S. PLoS One. 2013;8(2):e57311.
The nuclear receptor LXRalpha controls the functional specialization of splenic - The nuclear receptor LXRalpha controls the functional specialization of splenic
A-Gonzalez N, Guillen JA, Gallardo G, Diaz M, de la Rosa JV, Hernandez IH, Casanova-Acebes M, Lopez F, Tabraue C, Beceiro S, Hong C, Lara PC, Andujar M, Arai S, Miyazaki T, Li S, Corbi AL, Tontonoz P, Hidalgo A, Castrillo A. Nat Immunol. 2013 Aug;14(8):831-9.
Genome-wide analysis of LXRalpha activation reveals new transcriptional networks - Genome-wide analysis of LXRalpha activation reveals new transcriptional networks
Feldmann R, Fischer C, Kodelja V, Behrens S, Haas S, Vingron M, Timmermann B, Geikowski A, Sauer S. Nucleic Acids Res. 2013 Apr 1;41(6):3518-31.
Targeting GGTase-I activates RHOA, increases macrophage reverse cholesterol - Targeting GGTase-I activates RHOA, increases macrophage reverse cholesterol
Khan OM, Akula MK, Skalen K, Karlsson C, Stahlman M, Young SG, Boren J, Bergo MO. Circulation. 2013 Feb 19;127(7):782-90.
The liver X receptor pathway is highly upregulated in rheumatoid arthritis - The liver X receptor pathway is highly upregulated in rheumatoid arthritis
Asquith DL, Ballantine LE, Nijjar JS, Makdasy MK, Patel S, Wright PB, Reilly JH, Kerr S, Kurowska-Stolarska M, Gracie JA, McInnes IB. Ann Rheum Dis. 2013 Dec;72(12):2024-31.
Activation of liver X receptor suppresses the production of the IL-12 family of - Activation of liver X receptor suppresses the production of the IL-12 family of
Canavan M, McCarthy C, Larbi NB, Dowling JK, Collins L, O'Sullivan F, Hurley G, Murphy C, Quinlan A, Moloney G, Darby T, MacSharry J, Kagechika H, Moynagh P, Melgar S, Loscher CE. Innate Immun. 2014 Oct;20(7):675-87.
Human atherosclerotic plaque alternative macrophages display low cholesterol - Human atherosclerotic plaque alternative macrophages display low cholesterol
Chinetti-Gbaguidi G, Baron M, Bouhlel MA, Vanhoutte J, Copin C, Sebti Y, Derudas B, Mayi T, Bories G, Tailleux A, Haulon S, Zawadzki C, Jude B, Staels B. Circ Res. 2011 Apr 15;108(8):985-95.