All lanes : Anti-LYAR antibody [EPR14352] (ab181261) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : Ramos cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution
Anti-LYAR antibody [EPR14352] (ab181261) at 1/10000 dilution + A431 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution
Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling LYAR with ab181261 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution and counter-stained with DAPI (blue).