Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab182138 at 1/60 dilution (Lane 1). Lane 2: Negative control.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling LYAR with ab182138 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI (blue).
All lanes : Anti-LYAR antibody [EPR14352-9] (ab182138) at 1/20000 dilutionLane 1 : Ramos cell lysateLane 2 : Jurkat cell lysateLane 3 : A431 cell lysateLane 4 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution