ICC/IF image of ab106655 stained NIH-3T3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab106655 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Lane 1 : Anti-LZTR1 antibody (ab106655) at 1 µg/mlLane 2 : Anti-LZTR1 antibody (ab106655) at 2 µg/mlLane 1 : Human heart tissue lysateLane 2 : Human heart tissue lysateLysates/proteins at 15 µg per lane.
ab106655, at 5 µg/ml, staining LZTR1 in Mouse heart tissue by Immunohistochemistry.