Anti-Macrophage Inflammatory Protein 3 alpha antibody
| Name | Anti-Macrophage Inflammatory Protein 3 alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab9829 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | unknown |
| Applications | IHC-F IHC-P WB ELISA B/N ICC/IF ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Highly pure (>98%) recombinant hMIP-3-alpha (human macrophage inflammatory protein-3 alpha) |
| Description | Rabbit Polyclonal |
| Gene | CCL20 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab9829 (1µg/ml) staining Macrophage Inflammatory Protein 3 alpha in human tonsil (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of vesicles.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunohistochemical analysis of pancreatic ductal adenocarcinoma tissue, staining Macrophage Inflammatory Protein 3 alpha with ab9829. Antigen retrieval was performed by heat mediation in citrate buffer (pH 6.0). Tissue was blocked with 1% BSA for 10 min. The samples incubated overnight with primary antibody (1/40). Staining was detected using DAB. Sections were counterstained with hematoxylin.
Product References
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CCR6 recruits regulatory T cells and Th17 cells to the kidney in - CCR6 recruits regulatory T cells and Th17 cells to the kidney in
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C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the - C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the
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