Anti-MAD2L1 binding protein antibody [EPR9584]

• Supplier: Abcam
• Catalog: ab150363
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9584
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
• Description: Rabbit Monoclonal
• Gene: MAD2L1BP
• Conjugate: Unconjugated

Product images

ab150363 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab150363 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-MAD2L1 binding protein antibody [EPR9584] (ab150363) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : A431 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin embedded Human brain tissue labelling MAD2L1 binding protein with ab150363 antibody at a dilution of 1/100.

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labelling MAD2L1 binding protein with ab150363 antibody at a dilution of 1/100.