All lanes : Anti-MAF1 antibody (ab95971) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : MOLT4 whole cell lysateLysates/proteins at 30 µg per lane.
Immunoflourescent analysis of paraformaldehyde-fixed HeLa cells using ab95971 at 1/200 dilution.
ab95971 staining MAF1 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/100 in diluent) for 24 hours at 4°C. ab6721 goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview