Immunoprecipitation analysis of human testis tissue lysate labeling MAGEA9 using ab191568 at 1/100 dilution (Left Lane). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Right Lane: PBS instead of human testis tissue lysate.
Anti-MAGEA9 [EPR16258] antibody (ab191568) at 1/1000 dilution + K562 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometric analysis of 562 cells labeling MAGEA9 using ab191568 at 1/350 dilution (red) or rabbit monoclonal IgG as isotype control (green). Secondary antibody Goat anti rabbit IgG (FITC). Cells were fixed in 2% paraformaldehyde.
All lanes : Anti-MAGEA9 [EPR16258] antibody (ab191568) at 1/1000 dilutionLane 1 : Human testis lysateLane 2 : Human melanoma lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution