Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and nucleoli. Recommended concentration of ab122118 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-MAGEE2 antibody (ab122118) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cellsdeveloped using the ECL technique
ab122118, at 1/50 dilution, staining MAGEE2 in paraffin embedded Human colon tissue by Immunohistochemistry.