Anti-Mannosidase II antibody - Golgi Marker

• Supplier: Abcam
• Catalog: ab12277
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF
• Species Reactivities: Human, Monkey
• Antigen: Synthetic peptide to internal sequence (Human) conjugated to KLH
• Description: Rabbit Polyclonal
• Gene: MAN2A1
• Conjugate: Unconjugated

Product images

COS7 cells were fixed with cold methanol for 10 minutes followed by incubation with ab12277 (1/200) in 5% normal goat serum. FITC-conjugated goat anti-rabbit was used to visualise bound rabbit antibodies. Nuclei were stained with DAPI.

ICC/IF image of ab12277 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12277, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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