Anti-MAP1LC3A antibody [EP1528Y]

• Supplier: Abcam
• Catalog: ab52628
• Prices: $397.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1528Y
• Applications: WB IP ICC/IF IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues on the N-terminus of human MAPILC3A was used as an immogen
• Description: Rabbit Monoclonal
• Gene: MAP1LC3A
• Conjugate: Unconjugated

Product images

Anti-MAP1LC3A antibody [EP1528Y] (ab52628) at 1/50000 dilution + Fetal brain lysate at 10 µgSecondarygoat anti-rabbit HRP labelled at 1/2000 dilution

ab52628, at a 1/50 dilution, staining Human MAPILC3A in brain using Immunohistochemistry, Paraffin Embedded Tissue.

IHC image of ab52628 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52628, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Expression of autophagy-related proteins according to androgen receptor and HER-2 - Expression of autophagy-related proteins according to androgen receptor and HER-2

Kim JY, Jung WH, Koo JS. PLoS One. 2014 Aug 20;9(8):e105666.

ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery. - ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery.

Jung CH, Jun CB, Ro SH, Kim YM, Otto NM, Cao J, Kundu M, Kim DH. Mol Biol Cell. 2009 Apr;20(7):1992-2003.