Overlay histogram showing SHSY-5Y cells stained with ab52768 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52768, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768) at 1/10000 dilution + Hela cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/19190_ab52768_1.jpg)
Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab52768) at 1/10000 dilution + Hela cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution
Immunohistochemical staining of MAP1LC3A in paraffin embedded human breast carcinoma using ab52768 at a 1/100 dilution.Immunohistochemical staining of MAP1LC3A in paraffin embedded human breast carcinoma using ab52768 at a 1/100 dilution.