Anti-MAP4K3 antibody (ab103481) at 1/100 dilution + HepG2 cell line lysates at 35 µgdeveloped using the ECL technique
ab103481 at 1/50 dilution staining MAP4K3 in formalin-fixed and paraffin-embedded brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised DAB.
ICC/IF image of ab103481 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103481 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.