ab53358 staining MAPRE1 in Hela cells (red). Cells were fixed in methanol, permeabilised with 0.5% Triton X100/ PBS and counterstained both with DAPI - to highlight the nucleus (blue) - and an anti-tubulin antibody (green). Please refer to abreview for further experimental details.See Abreview
![Anti-MAPRE1 antibody [KT51] (ab53358) at 1/1000 dilution + lysate prepared from HeLa cells at 50 µgSecondaryRabbit Anti-Rat IgG H&L (HRP) (ab6734) at 1/2000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/19536_MAPRE1-Primary-antibodies-ab53358-10.jpg)
Anti-MAPRE1 antibody [KT51] (ab53358) at 1/1000 dilution + lysate prepared from HeLa cells at 50 µgSecondaryRabbit Anti-Rat IgG H&L (HRP) (ab6734) at 1/2000 dilutiondeveloped using the ECL technique
![Overlay histogram showing HeLa cells stained with ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53358, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/19537_MAPRE1-Primary-antibodies-ab53358-11.jpg)
Overlay histogram showing HeLa cells stained with ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53358, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.