Anti-MARCKS antibody (ab51100) at 1/2000 dilution + 293 cells, lysed in RIPA buffer containing minicomplete protease inhibitors at 30 µgSecondaryHRP conjugated goat anti-rabbitdeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 63 kDa (why is the actual band size different from the predicted?)Additional bands at : 19 kDa. We are unsure as to the identity of these extra bands.Exposure time : 30 secondsThis image is courtesy of an anonymous AbreviewSee Abreview