Anti-MARCKS antibody [EP1446Y]

• Supplier: Abcam
• Catalog: ab52616
• Prices: $391.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1446Y
• Applications: ICC/IF ICC/IF WB ICC/IF FC IHC-P
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues on human MARCKS
• Description: Rabbit Monoclonal
• Gene: MARCKS
• Conjugate: Unconjugated

Product images

ICC/IF image of ab52616 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab52616 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MARCKS antibody [EP1446Y] (ab52616) at 1/10000 dilution + Human fetal brain lysate at 10 µgSecondaryGoat anti-rabbit HRP antibody at 1/2000 dilution

Ab52616 at 1/100 dilution staining human brain tissue; paraffin embedded.Ab52616 at 1/100 dilution staining human brain tissue; paraffin embedded.

Overlay histogram showing HeLa cells stained with ab52616 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52616, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Product References

Functional characterization of the tumor-suppressor MARCKS in colorectal cancer - Functional characterization of the tumor-suppressor MARCKS in colorectal cancer

Bickeboller M, Tagscherer KE, Kloor M, Jansen L, Chang-Claude J, Brenner H, Hoffmeister M, Toth C, Schirmacher P, Roth W, Blaker H. Oncogene. 2015 Feb 26;34(9):1150-9.

Combined PKC and MEK inhibition in uveal melanoma with GNAQ and GNA11 mutations. - Combined PKC and MEK inhibition in uveal melanoma with GNAQ and GNA11 mutations.

Chen X, Wu Q, Tan L, Porter D, Jager MJ, Emery C, Bastian BC. Oncogene. 2014 Sep 25;33(39):4724-34.

MARCKS regulates growth and radiation sensitivity and is a novel prognostic - MARCKS regulates growth and radiation sensitivity and is a novel prognostic

Jarboe JS, Anderson JC, Duarte CW, Mehta T, Nowsheen S, Hicks PH, Whitley AC, Rohrbach TD, McCubrey RO, Chiu S, Burleson TM, Bonner JA, Gillespie GY, Yang ES, Willey CD. Clin Cancer Res. 2012 Jun 1;18(11):3030-41.

Control of basal autophagy by calpain1 mediated cleavage of ATG5. - Control of basal autophagy by calpain1 mediated cleavage of ATG5.

Xia HG, Zhang L, Chen G, Zhang T, Liu J, Jin M, Ma X, Ma D, Yuan J. Autophagy. 2010 Jan;6(1):61-6. Epub 2010 Jan 13.

Coding microsatellite instability analysis in microsatellite unstable small - Coding microsatellite instability analysis in microsatellite unstable small

Michel S, Kloor M, Singh S, Gdynia G, Roth W, von Knebel Doeberitz M, Schirmacher P, Blaker H. Mol Carcinog. 2010 Feb;49(2):175-82.

PhosphoMARCKS drives motility of mouse melanoma cells. - PhosphoMARCKS drives motility of mouse melanoma cells.

Chen X, Rotenberg SA. Cell Signal. 2010 Jul;22(7):1097-103.