All lanes : Anti-MARCO antibody (ab102124) at 1 µg/mlLane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 2 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissueLane 4 : Lung (Human) Tissue LysateLane 5 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699) with Immunizing peptide at 1 µg/mlLane 6 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871) with Immunizing peptide at 1 µg/mlLane 7 : Bone Marrow (Human) Tissue Lysate - adult normal tissue with Immunizing peptide at 1 µg/mlLane 8 : Lung (Human) Tissue Lysate with Immunizing peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 51 kDa (why is the actual band size different from the predicted?)Additional bands at : 56 kDa,80 kDa. We are unsure as