All lanes : Anti-MARK 1+2+3+4 antibody (ab74131) at 1/500 dilutionLane 1 : Extracts from COS-7 cellsLane 2 : Extracts from COS-7 cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab74131, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Immunofluorescence analysis of HeLa cells, using ab74131 at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.