Immunofluorescent analysis of HeLa cells (acetone-fixed) labeling MARS with ab180497 at 1/250 dilution (green) followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution and DAPI staining (blue).
All lanes : Anti-MARS antibody [EPR9872] (ab180497) at 1/10000 dilutionLane 1 : HepG2 lysateLane 2 : HeLa lysateLane 3 : SW480 lysateLane 4 : Caco2 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling MARS with ab180497 at 1/50 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.
Western blot analysis of immunoprecipitation pellet from HepG2 lysate immunoprecipitated using ab180497 at 1/40 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.