Anti-MASPIN antibody (ab22354) at 1 µg/ml + Prostate (Human) Whole Cell Lysate - tumor tissue (ab30305) at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutionPerformed under reducing conditions.
ab22354 staining MASPIN in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in TRIS-EDTA Buffer pH 9.0 and permeabilized with a wash buffer with Tween. The primary antibody was diluted 1/2000 and incubated with the sample for 30 minutes at 20°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody.See Abreview
All lanes : Anti-MASPIN antibody (ab22354) at 1 µg/mlLane 1 : Human Prostate Tumor Tissue Lysate at 20 ugLane 2 : Human Prostate Tumor Tissue Lysate at 20 ug with Human MASPIN peptide (ab24350) at 1 µg/mlSecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution Performed under reducing conditions.