All lanes : Anti-Mast Cell Tryptase antibody (ab150034) at 1 µg/mlLane 1 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 2 : Pancreas (Human) Tissue Lysate - adult normal tissue (ab29816)Lane 3 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)Lane 4 : Stomach (Human) Whole Cell Lysate - tumor tissue (ab29678)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.