Anti-Mast Cell Tryptase antibody [AA1]

• Supplier: Abcam
• Catalog: ab2378
• Prices: $400.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: AA1
• Applications: ICC/IF WB IHC-P IHC-F ICC/IF ICC/IF IHC ELISA
• Species Reactivities: Mouse, Rat, Human, Cat, Dog, Monkey
• Antigen: Purified human lung Tryptase
• Description: Mouse Monoclonal
• Gene: TPSAB1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Mast Cell Tryptase antibody [AA1] (ab2378) at 5 µg/mlLane 1 : Lung (Human) Whole Cell Lysate - adult normal tissue (ab30281)Lane 2 : Tonsil (Human) Whole Cell Lysate - adult normal tissue (ab29615)Lane 3 : Skin (Human) Tissue Lysate - adult normal tissue (ab30166)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ab2378 staining formalin fixed paraffin-embedded human colon tissue sections cut at 1 micron.  The section was subjected to heat mediated antigen retrieval in Dako Target Retrieval Solution and permeabilized in Triton-X prior to blocking in 6% BSA for 2 hours at 24°C.  The primary anitbody was diluted 1/250 and incubated with the sample for 16 hours at 5°C.  The secondary antibody was ab7064 (diluted 1/2000) and the slide was counterstained with DAPI.See Abreview

ab2378 staining Mast Cell Tryptase in Human nasal polyp and tonsil tissue sections by Immunohistochemistry (resin-embedded sections). Tissue was fixed with acetone and inhibitors and embedded in GMA resin. Samples were incubated with primary antibody (1/2000 in Tris buffered saline) for 18 hours at 21°C. A Biotin-conjugated Rabbit anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview

ab2378 staining Mast Cell Tryptase in Human skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol, permeabilized with 0.1% Saponin/PBS and blocked with 4% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100) for 14 hours at 4°C. A Biotin-conjugated Horse anti-mouse polyclonal (10 µg/ml) was used as the secondary antibody.See Abreview

ab2378 staining Mast Cell Tryptase in Human mast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with Saponin 0.1% in PBS and blocked with 4% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 14 hours at 4°C. A Texas Red®-conjugated Goat anti-mouse polyclonal (1/100) was used as the secondary antibody.See Abreview

Product References

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