All lanes : Anti-Matriptase 3 antibody - Catalytic domain (ab42462) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (treated with SF)Lane 2 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (treated with TPA)Lane 3 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (treated with IL1-beta)Lane 4 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (treated with TNF-alpha)Lane 5 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (treated with RA)