Anti-MAX antibody [73C5a] - ChIP Grade
| Name | Anti-MAX antibody [73C5a] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab53570 |
| Prices | $384.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 73C5a |
| Applications | FC ChIP WB DB |
| Species Reactivities | Human |
| Antigen | Recombinant human MAX |
| Description | Mouse Monoclonal |
| Gene | MAX |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-MAX antibody [73C5a] - ChIP Grade (ab53570) + immunised recombinant protein](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/19977_ab53570.gif)
Anti-MAX antibody [73C5a] - ChIP Grade (ab53570) + immunised recombinant protein
![Overlay histogram showing A549 cells stained with ab53570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53570, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/19978_MAX-Primary-antibodies-ab53570-1.jpg)
Overlay histogram showing A549 cells stained with ab53570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53570, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased - c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased
Xue G, Yan HL, Zhang Y, Hao LQ, Zhu XT, Mei Q, Sun SH. Oncogene. 2015 Mar 12;34(11):1393-406.
Inhibition of miR-193a expression by Max and RXRalpha activates K-Ras and PLAU to - Inhibition of miR-193a expression by Max and RXRalpha activates K-Ras and PLAU to
Iliopoulos D, Rotem A, Struhl K. Cancer Res. 2011 Aug 1;71(15):5144-53.
STAT3 activation of miR-21 and miR-181b-1 via PTEN and CYLD are part of the - STAT3 activation of miR-21 and miR-181b-1 via PTEN and CYLD are part of the
Iliopoulos D, Jaeger SA, Hirsch HA, Bulyk ML, Struhl K. Mol Cell. 2010 Aug 27;39(4):493-506.