All lanes : Anti-MCK10 antibody (ab135643) at 1/50 dilutionLane 1 : 293 cells lysatesLane 2 : 293 cells transfected with MCK10 gene lysateLysates/proteins at 2 µg per lane.
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue labelling MCK10 with ab135643 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunofluorescent analysis of 293 cells labelling MCK10 with ab135643 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of 293 cells labelling MCK10 with ab135643 at 1/10 dilution (right histogram) compared to a negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.