Anti-MCSF antibody

• Supplier: Abcam
• Catalog: ab9693
• Prices: $390.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: unknown
• Applications: WB ELISA B/N ELISA IHC-P
• Species Reactivities: Human
• Antigen: Highly pure (>98%) recombinant hM-CSF (human M-CSF)
• Description: Rabbit Polyclonal
• Gene: CSF1
• Conjugate: Unconjugated

Product images

ab9693 staining MCSF in human cervical squamous cell carcinoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

ab9693 staining MCSF in human cervical squamous cell carcinoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

Anti-MCSF antibody (ab9693) at 0.2 µg/ml + Active MCSF full length protein (ab9694) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute

All lanes : Anti-MCSF antibody (ab9693) at 1/500 dilution (for 12 hours at 4°C)Lane 1 : Human Kidney whole cell lysateLane 2 : Human Kidney whole cell lysate transfected with LFABPLysates/proteins at 15 µg per lane.SecondaryAn IRDye®800CW-conjugated Goat anti-rabbit IgG polyclonal at 1/15000 dilutionPerformed under non-reducing conditions.Observed band size : 70 kDa (why is the actual band size different from the predicted?)This image is courtesy of an Abreview submitted by Valentina MarcelliSee Abreview

Standard curve for MCSF (Analyte: ab62015); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [21] to MCSF (ab18645) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCSF (ab9693) at 0.1µg/ml.

Product References

Biomarkers and endosalpingiosis in the ovarian and tubal microenvironment of - Biomarkers and endosalpingiosis in the ovarian and tubal microenvironment of

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Characterisation of a novel Fc conjugate of macrophage colony-stimulating factor. - Characterisation of a novel Fc conjugate of macrophage colony-stimulating factor.

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M-CSF accelerates orthodontic tooth movement by targeting preosteoclasts in mice. - M-CSF accelerates orthodontic tooth movement by targeting preosteoclasts in mice.

Brooks PJ, Heckler AF, Wei K, Gong SG. Angle Orthod. 2011 Mar;81(2):277-83.

Imatinib sensitivity as a consequence of a CSF1R-Y571D mutation and CSF1/CSF1R - Imatinib sensitivity as a consequence of a CSF1R-Y571D mutation and CSF1/CSF1R

Chase A, Schultheis B, Kreil S, Baxter J, Hidalgo-Curtis C, Jones A, Zhang L, Grand FH, Melo JV, Cross NC. Leukemia. 2009 Feb;23(2):358-64.