Anti-MCSF antibody
| Name | Anti-MCSF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab66236 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | FC ELISA WB |
| Species Reactivities | Human, Bovine, Dog, Chimpanzee, Monkey |
| Antigen | Recombinant fragment with tag: EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK, corresponding to amino acids 33-132 of Human MCSF Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | CSF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-MCSF antibody (ab66236) at 1 µg/mlLane 1 : MCSF transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 50 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
 used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 4% paraformaldehyde (10 min) and permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/20668_MCSF-Primary-antibodies-ab66236-1.jpg)
Overlay histogram showing HL60 cells stained with ab66236 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66236, 1 µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-Mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1](ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 4% paraformaldehyde (10 min) and permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.