Anti-MD2 antibody

• Supplier: Abcam
• Catalog: ab24182
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: FC IHC-F WB ICC/IF ICC/IF IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to amino acids near the middle region of human MD2, between residues 110 and 160
• Description: Rabbit Polyclonal
• Gene: LY96
• Conjugate: Unconjugated

Product images

Anti-MD2 antibody (ab24182) at 1 µg/ml (mouse spleen lysate)

ab24182 at 2µg/ml staining of MD2 in rat spleen cells by IHC

    ab 24182 at a 1/100 dilution staining Rat mixed primary glial cell culture by FACS analysis. The antibody was incubated with the cells for 45 minutes and then detected using an Alexa-Fluor ® 488 Goat anti-rabbit antibody. For the negative control, primary antibody was omitted.This image is courtesy of an Abreview by Nancy Nutile-McMenemy submitted on 16 March 2006.See Abreview

ab24182 at a 1/200 dilution staining rat spinal cord tissue sections from a 4% PFA transcardially perfused animal by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and incubated with the antibody for 18 hours. Bound antibody was detected using an HRP conjugated goat anti-mouse polyclonal antibody. This image is courtesy of an Abreview submitted by Nancy Nutile-McMenemy.See Abreview

All lanes : Anti-MD2 antibody (ab24182) at 2 µg/ml (in 5% milk in TBS/T 0.1% (blocking buffer) for 12 hours at 4°C)Lane 1 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hoursLane 2 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and LPS (50 ng/ml) for 10 minutesLane 3 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and ethanol (50 mM) for 10 minutesLane 4 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hoursLane 5 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and LPS (50 ng/ml) for 10 minutesLane 6 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and ethanol (50 mM) for 10 minutesLysates/proteins at 40 µg per lane.SecondaryAn HRP-conjugated Goat anti-rabbit IgG at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Flow cytometry analysis of Human corneal epithelial cell lines, HCEC and HCET, staining MD2 with ab24182. Cells were either unstimulated (unstim - black line) or stimulated (+IFN-γ - grey histogram), fixed in paraformaldehyde and incubated with primary antibody for 45 minutes at 4°C. A fluorescent-conjugated secondary antibody was used to detect staining.

Product References

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