Anti-MDC1 antibody
| Name | Anti-MDC1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab11169 |
| Prices | $392.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Chimpanzee, Monkey, Ape |
| Antigen | Synthetic peptide, which represents a portion of the human Mediator of DNA Damage Checkpoint Protein 1 encoded within exon 5 (LocusLink ID 9656) |
| Description | Rabbit Polyclonal |
| Gene | MDC1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab11169 staining MDC1 in HCT116 cells treated with GANT 61 (ab120904), by ICC/IF. Increase in MDC1 expression correlates with increased concentration of GANT 61, as described in literature.The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120904 (GANT 61) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11169 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
ab11169 staining MDC1 in human cervical cancer cells (HeLa) by ICC/IF (immunocytochemistry/immunofluorescence - left image). Cells were paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 25°C. The primary antibody (1/200) was incubated with the sample for 12 hours at 4°C. An Alexa Fluor 594®-conjugated Chicken anti-rabbit polyclonal (1/2000) was used as the secondary. The nuclei were stained with DAPI (right image).See Abreview
Immunofluorescence analysis of A549 cells before (left) and after ionizing radiation (right), staining MDC1 with ab11169. Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% BSA for 30 minutes at room temperature. Cells were incubated with primary antibody for 1 hour at room temperature. A Cy2®-conjugated anti-rabbit IgG was used as the secondary antibody.
Product References
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CBX4-mediated SUMO modification regulates BMI1 recruitment at sites of DNA - CBX4-mediated SUMO modification regulates BMI1 recruitment at sites of DNA
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