Anti-MDC1 antibody

• Supplier: Abcam
• Catalog: ab11171
• Prices: $390.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IP IHC-P
• Species Reactivities: Human, Chimpanzee
• Antigen: Synthetic peptide, which represents a portion of the human Mediator of DNA Damage Checkpoint Protein 1 encoded within exon 8 (LocusLink ID 9656)
• Description: Rabbit Polyclonal
• Gene: MDC1
• Conjugate: Unconjugated

Product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MDC1 with ab11171 at 1/1000 (1µg/ml). Detection: DAB.

IHC image of MDC1 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11171, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ICC/IF image of ab11171 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11171, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Ubiquitin-activating enzyme UBA1 is required for cellular response to DNA damage. - Ubiquitin-activating enzyme UBA1 is required for cellular response to DNA damage.

Moudry P, Lukas C, Macurek L, Hanzlikova H, Hodny Z, Lukas J, Bartek J. Cell Cycle. 2012 Apr 15;11(8):1573-82.

53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of - 53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of

Lukas C, Savic V, Bekker-Jensen S, Doil C, Neumann B, Pedersen RS, Grofte M, Chan KL, Hickson ID, Bartek J, Lukas J. Nat Cell Biol. 2011 Mar;13(3):243-53.

The NuRD chromatin-remodeling complex regulates signaling and repair of DNA - The NuRD chromatin-remodeling complex regulates signaling and repair of DNA

Smeenk G, Wiegant WW, Vrolijk H, Solari AP, Pastink A, van Attikum H. J Cell Biol. 2010 Sep 6;190(5):741-9.