All lanes : Anti-MDH1 antibody - C-terminal (ab135529) at 1/50 dilutionLane 1 : HL60 cell line lysateLane 2 : HepG2 cell line lysateLysates/proteins at 35 µg per lane.
All lanes : Anti-MDH1 antibody - C-terminal (ab135529) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with the MDH1 geneLysates/proteins at 2 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labelling MDH1 with ab135529 at 1/50 dilution, followed by a peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometry analysis of HL60 cells labelling MDH1 with ab135529 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.