Anti-MDM2 (phospho S166) antibody
| Name | Anti-MDM2 (phospho S166) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58532 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic phosphopeptide derived from human MDM2 around the phosphorylation site of serine 166 (A-I-SP-E-T) |
| Description | Rabbit Polyclonal |
| Gene | MDM2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-MDM2 (phospho S166) antibody (ab58532) at 1/500 dilutionLane 1 : COS7 cell extractLane 2 : COS7 cell extract with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.
ICC/IF image of ab58532 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58532, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.