Anti-MDM2 antibody
| Name | Anti-MDM2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58530 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB IHC-P ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic non phosphopeptide derived from human MDM2 around the phosphorylation site of serine 166 (A-I-SP-E-T) |
| Description | Rabbit Polyclonal |
| Gene | MDM2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![MDM2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to MDM2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab58530.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 85kDa; MDM2](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/20869_ab58530-208248-IPVI003ab5853020mMod.jpg)
MDM2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to MDM2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab58530.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 85kDa; MDM2
All lanes : Anti-MDM2 antibody (ab58530)Lane 1 : COS7 cell lysateLane 2 : COS7 cell lysate with blocking peptide
ab58530 staining MDM2 in NIH/3T3 cells by Immunocytochemistry/ Immunofluorescence. Shown both in the absence (left) and presence (right) of MDM2 blocking peptide.
Product References
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Liu XH, Yao S, Levine AC, Kirschenbaum A, Pan J, Wu Y, Qin W, Collier L, Bauman WA, Cardozo CP. J Androl. 2012 Nov-Dec;33(6):1216-23.
Mdm2 promotes systemic lupus erythematosus and lupus nephritis. - Mdm2 promotes systemic lupus erythematosus and lupus nephritis.
Allam R, Sayyed SG, Kulkarni OP, Lichtnekert J, Anders HJ. J Am Soc Nephrol. 2011 Nov;22(11):2016-27.