Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling MDM2 with ab137413 at 1/500 (Green). Alpha-tubulin filaments were also labelled (red).
All lanes : Anti-MDM2 antibody (ab137413) at 1/1000 dilutionLane 1 : NIH 3T3 whole cell lysateLane 2 : JC whole cell lysateLane 3 : BCL-1 whole cell lysateLysates/proteins at 30 µg per lane.
Anti-MDM2 antibody (ab137413) at 1/5000 dilution + HeLa whole cell lysate at 30 µg
Immunohistochemical analysis of paraffin-embedded A549 xenograft, labelling MDM2 using ab137413 at 1/100 dilution.