Anti-MDM2 antibody
| Name | Anti-MDM2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab87134 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Horse, Bovine, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human MDM2 |
| Description | Rabbit Polyclonal |
| Gene | MDM2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-MDM2 antibody (ab87134) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466)SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab87134 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87134 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.