Anti-ME1 antibody
| Name | Anti-ME1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab97445 |
| Prices | $390.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human, Bovine |
| Antigen | Recombinant fragment corresponding to a region within amino acids 161-489 of Human ME1 (NP_002386) |
| Description | Rabbit Polyclonal |
| Gene | ME1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-ME1 antibody (ab97445) at 1/500 dilutionLane 1 : A431 whole cell lysateLane 2 : HeLa S3 whole cell lysateLysates/proteins at 30 µg per lane.
Immunofluorescence analysis of ME1 in paraformaldehyde fixed HeLa, using ab97445 at a 1/200 dilution. Lower image: merged with DNA probe.
IHC image of ab97445 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97445, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Metabolic intervention on lipid synthesis converging pathways abrogates prostate - Metabolic intervention on lipid synthesis converging pathways abrogates prostate
Fritz V, Benfodda Z, Henriquet C, Hure S, Cristol JP, Michel F, Carbonneau MA, Casas F, Fajas L. Oncogene. 2013 Oct 17;32(42):5101-10.
Cytosolic malic enzyme 1 (ME1) mediates high fat diet-induced adiposity, - Cytosolic malic enzyme 1 (ME1) mediates high fat diet-induced adiposity,
Al-Dwairi A, Pabona JM, Simmen RC, Simmen FA. PLoS One. 2012;7(10):e46716.
Dietary soy protein induces hepatic lipogenic enzyme gene expression while - Dietary soy protein induces hepatic lipogenic enzyme gene expression while
Hakkak R, Al-Dwairi A, Fuchs GJ, Korourian S, Simmen FA. Genes Nutr. 2012 Oct;7(4):549-58. Epub 2012 Apr 24.