Anti-ME1 antibody (ab87974) at 5 µg/ml + recombinant human ME1 protein with a proprietary tag (the immunogen) at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Anti-ME1 antibody (ab87974) at 5 µg/ml + HepG2 cell lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
All lanes : Anti-ME1 antibody (ab87974) at 5 µg/mlLane 1 : ME1 transfected lysate from 293T cell lineLane 2 : Non transfected lysate from 293T cell lineLysates/proteins at 50 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
ab87974, at 0.5 µg/ml, staining ME1 in formalin fixed, paraffin embedded human tonsil tissue sections by Immunoperoxidase/Immunohistochemistry.
ab87974, detecting recombinant tagged ME1 by sandwich ELISA. The detection limit is approximately 1 ng/ml as a capture antibody.