Anti-MeCP2 antibody - ChIP Grade
| Name | Anti-MeCP2 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab2828 |
| Prices | $395.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IP WB EMSA IHC-F IHC-P ICC/IF IHC Radioimmunoprecipitation ChIP |
| Species Reactivities | Mouse, Rat, Human, Primate, Amphibians, Monkey |
| Antigen | Synthetic peptide corresponding to Mouse MeCP2 aa 1-15 |
| Description | Rabbit Polyclonal |
| Gene | MECP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 5 µg of ab2828 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
ab2828 staining MeCP2 (green) in mouse hippocampus CA1 tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.1% Triton X-100 and blocked with 3% serum for 30 minutes at 23°C. The sample was incubated with primary antibody (1/200 in PBS + 3% BSA + 0.1% Triton X-100) at 4°C for 12 hours. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) polyclonal (1/1000) was used as the secondary antibody. Counterstained neuronal marker mouse anti-NeuN (red).See Abreview
Product References
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